Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Inhibition rate of cancer cell under taking TGL in Kunming mice. (a) The tumor of mice bearing S180 and H22 cells. (b) Tumor weight. (c) Tumor inhibition rate. n = 12. The different lowercase and uppercase letters means significant difference between S180 and H22 cells respectively, p < 0.05.

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques: Inhibition

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Effect of TGL on immune organ and organ index in mice. (a) Spleen and thymus of mice bearing S180 and H22 cells. (b) Spleen index. (c) Thymus index. n = 12. The different lowercase and uppercase letters means significant difference between S180 and H22 cells respectively, p < 0.05.

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques:

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Effect of TGL on the antioxidant components in the serum of mice bearing S180 and H22 cells. (a) SOD (mmol/L). (b) CAT (mmol/L). (c) MDA (mmol/L). n = 12. The different lowercase and uppercase letters means significant difference between S180 and H22 cells respectively, p < 0.05.

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques:

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Expression of Bcl-2 and Bax protein in S180 and H22 tumor and gut by TGL treatment. (a) Expression of Bcl-2 and Bax protein in S180 and H22 tumor. (b) Expression of Bcl-2 and Bax protein in gut. n = 12. The different lowercase and uppercase letters means significant difference between Bcl-2 and Bax protein expression respectively, p < 0.05. Original images of western blot were shown in .

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques: Expressing, Western Blot

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Sequencing data and the alpha diversity in each group of mice (n = 12).

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques: Sequencing

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Distribution of gut microbiota at different level. (a) Analysis of the composition of bacteria at the genus level of S180 tumor-bearing mice. (b) Analysis of the composition of bacteria at the genus level of H22 tumor-bearing mice. (c) 3D-PCoA of the gut microbiota of S180 tumor-bearing mice. (d) 3D-PCoA of the gut microbiota of H22 tumor-bearing mice. n = 12.

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques: Bacteria

Journal: Heliyon

Article Title: Triterpenoids of Ganoderma lucidum inhibited S180 sarcoma and H22 hepatoma in mice by regulating gut microbiota

doi: 10.1016/j.heliyon.2023.e16682

Figure Lengend Snippet: Line chart of species classification box. (a) Gut microbiota of S180 tumor-bearing mice. (b) Gut microbiota of H22 tumor-bearing mice.

Article Snippet: S180 mouse ascites sarcoma cells and H22 mouse ascites liver cancer cells were provided by Lanzhou University Institute of Pharmacology and purchased from ATCC.

Techniques:

Journal: Scientific Reports

Article Title: Anti-tumor effect of β-glucan from Lentinus edodes and the underlying mechanism

doi: 10.1038/srep28802

Figure Lengend Snippet: (A ) Cell viability of H8, LO2 and 293T cells after treatment with LNT for 48 h determined by MTT assay. ( B ) Cell viability of Hela and S-180 cells after treatment with LNT for 48 h determined by MTT assay. * p < 0.05 and ** p < 0.001 versus the control (0 μg/mL). Hela ( C ) and S-180 ( E ) cells were respectively treated with LNT (200 μg/mL, 400 μg/mL) for indicated time intervals, and the whole cell extracts were prepared, 30 μg of which were resolved on 10% or 12% SDS-PAGE. p53, p21, Bax, Bcl-2 and caspase 3 were detected by western blot analysis using their specific antibodies with β-actin as the loading control. ( D , F ) The digital results were determined by quantitative densitometry. The results shown are representative of three independent experiments. * p < 0.05 and ** p < 0.001 versus the control. ( G ) Hela cells were transfected with p53 siRNA, and then treated with LNT (200 μg/mL) for indicated time intervals, and the whole cell extracts were prepared, 30 μg of which were resolved on 10% or 12% SDS-PAGE. p53, p21, Bax, Bcl-2 and caspase 3 were detected by western blot analysis using their specific antibodies with β-actin as the loading control. The blots are run under the same experimental conditions. ( H ) Hela cells were transfected with p53 siRNA, and then treated with or without LNT at desired concentration for 48 h. Cell viability was determined by MTT assay.

Article Snippet: Human renal epithelial cell line (293T), human cervical carcinoma cell line (Hela), human hepatoyte cell line (LO2) and Mouse sarcoma 180 cell line (S-180) were purchased from China center for typical culture collection (CCTCC).

Techniques: MTT Assay, Control, SDS Page, Western Blot, Transfection, Concentration Assay